@article{ISI:000389962500023,
 abstract = {Functionalization of quantum dots (QDs) with a single biomolecular tag
using traditional approaches in bulk solution has met with limited
success. DNA polyhedra consist of an internal void bounded by a
well-defined three-dimensional structured surface. The void can house
cargo and the surface can be functionalized with stoichiometric and
spatial precision. Here, we show that monofunctionalized QDs can be
realized by encapsulating QDs inside DNA icosahedra and functionalizing
the DNA shell with an endocytic ligand. We deployed the DNA-encapsulated
QDs for real-time imaging of three different endocytic ligands-folic
acid, galectin-3 (Gal3) and the Shiga toxin B-subunit (STxB).
Single-particle tracking of Gal3- or STxB-functionalized QD-loaded DNA
icosahedra allows us to monitor compartmental dynamics along endocytic
pathways. These DNA-encapsulated QDs, which bear a unique stoichiometry
of endocytic ligands, represent a new class of molecular probes for
quantitative imaging of endocytic receptor dynamics.},
 author = {Bhatia, Dhiraj and Arumugam, Senthil and Nasilowski, Michel and Joshi,
Himanshu and Wunder, Christian and Chambon, Valerie and Prakash, Ved and
Grazon, Chloe and Nadal, Brice and Maiti, Prabal K. and Johannes, Ludger
and Dubertret, Benoit and Krishnan, Yamuna},
 doi = {10.1038/NNANO.2016.150},
 eissn = {1748-3395},
 issn = {1748-3387},
 journal = {NATURE NANOTECHNOLOGY},
 month = {DEC},
 number = {12},
 orcid-numbers = {Maiti, Prabal/0000-0002-9956-1136
Wunder, Christian/0000-0001-9091-0080
Johannes, Ludger/0000-0002-2168-0004
Johannes, Ludger/0000-0002-2168-0004
BHATIA, DHIRAJ/0000-0002-1478-6417
Grazon, Chloe/0000-0002-4564-8738
Arumugam, Senthil/0000-0001-6733-4679
Krishnan, Yamuna/0000-0001-5282-8852},
 pages = {1112-1119},
 researcherid-numbers = {Maiti, Prabal/B-6335-2009
Wunder, Christian/K-8542-2017
Johannes, Ludger/W-8593-2019
Johannes, Ludger/M-9778-2017
BHATIA, DHIRAJ/Y-9699-2019
BHATIA, DHIRAJ/F-4736-2013
},
 times-cited = {77},
 title = {Quantum dot-loaded monofunctionalized DNA icosahedra for single-particle
tracking of endocytic pathways},
 unique-id = {ISI:000389962500023},
 volume = {11},
 year = {2016}
}