@article{ISI:000360953300024,
 abstract = {Viral capsids derived from an icosahedral plant virus widely used in
physical and nanotechnological investigations were fully dissociated
into dimers by a rapid change of pH. The process was probed in vitro at
high spatiotemporal resolution by time-resolved small-angle X-ray
scattering using a high brilliance synchrotron source. A powerful
custom-made global fitting algorithm allowed us to reconstruct the most
likely pathway parametrized by a set of stoichiometric coefficients and
to determine the shape of two successive intermediates by ab initio
calculations. None of these two unexpected intermediates was previously
identified in self-assembly experiments, which suggests that the
disassembly pathway is not a mirror image of the assembly pathway. These
findings shed new light on the mechanisms and the reversibility of the
assembly/disassembly of natural and synthetic virus-based systems. They
also demonstrate that both the structure and dynamics of an increasing
number of intermediate species become accessible to experiments.},
 author = {Law-Hine, Didier and Sahoo, Anil K. and Bailleux, Virginie and Zeghal,
Mehdi and Prevost, Sylvain and Maiti, Prabal K. and Bressanelli,
Stephane and Constantin, Doru and Tresset, Guillaume},
 doi = {10.1021/acs.jpclett.5b01478},
 issn = {1948-7185},
 journal = {JOURNAL OF PHYSICAL CHEMISTRY LETTERS},
 month = {SEP 3},
 number = {17},
 orcid-numbers = {Prevost, Sylvain/0000-0002-6008-1987
Constantin, Doru/0000-0001-5623-9633
Maiti, Prabal/0000-0002-9956-1136
Bressanelli, Stephane/0000-0002-0921-6727
Sahoo, Anil Kumar/0000-0001-7769-4774},
 pages = {3471-3476},
 researcherid-numbers = {Prevost, Sylvain/A-8740-2012
Constantin, Doru/H-8495-2013
Maiti, Prabal/B-6335-2009
},
 times-cited = {18},
 title = {Reconstruction of the Disassembly Pathway of an Icosahedral Viral Capsid
and Shape Determination of Two Successive Intermediates},
 unique-id = {ISI:000360953300024},
 volume = {6},
 year = {2015}
}