@article{ISI:000291876600001,
 abstract = {We review the current status of various aspects of biopolymer
translocation through nanopores and the challenges and opportunities it
offers. Much of the interest generated by nanopores arises from their
potential application to third-generation cheap and fast genome
sequencing. Although the ultimate goal of single-nucleotide
identification has not yet been reached, great advances have been made
both from a fundamental and an applied point of view, particularly in
controlling the translocation time, fabricating various kinds of
synthetic pores or genetically engineering protein nanopores with
tailored properties, and in devising methods (used separately or in
combination) aimed at discriminating nucleotides based either on ionic
or transverse electron currents, optical readout signatures, or on the
capabilities of the cellular machinery. Recently, exciting new
applications have emerged, for the detection of specific proteins and
toxins (stochastic biosensors), and for the study of protein folding
pathways and binding constants of protein-protein and protein-DNA
complexes. The combined use of nanopores and advanced micromanipulation
techniques involving optical/magnetic tweezers with high spatial
resolution offers unique opportunities for improving the basic
understanding of the physical behavior of biomolecules in confined
geometries, with implications for the control of crucial biological
processes such as protein import and protein denaturation. We highlight
the key works in these areas along with future prospects. Finally, we
review theoretical and simulation studies aimed at improving fundamental
understanding of the complex microscopic mechanisms involved in the
translocation process. Such understanding is a pre-requisite to fruitful
application of nanopore technology in high-throughput devices for
molecular biomedical diagnostics.},
 author = {Kumar, Hemant and Lansac, Yves and Glaser, Matthew A. and Maiti, Prabal
K.},
 doi = {10.1039/c0sm01517b},
 eissn = {1744-6848},
 issn = {1744-683X},
 journal = {SOFT MATTER},
 number = {13},
 orcid-numbers = {Kumar, Arun/0000-0001-8422-0219
Glaser, Matthew/0000-0002-8366-5598
Maiti, Prabal/0000-0002-9956-1136},
 pages = {5898-5907},
 researcherid-numbers = {Kumar, Hemant/F-3331-2013
Kumar, Arun/C-9149-2009
Glaser, Matthew/H-2345-2016
Maiti, Prabal/B-6335-2009},
 times-cited = {29},
 title = {Biopolymers in nanopores: challenges and opportunities},
 unique-id = {ISI:000291876600001},
 volume = {7},
 year = {2011}
}